Onion variety NUN 2002 ON

ABSTRACT

The invention relates to the field of  Allium  in particular to a new variety of onion designated NUN 2002 ON as well as plants, seeds and bulbs thereof.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of onion variety NUN 2002 ON (or NUN2002 or NUN 2002 F1 or NUN 2002 hybrid or 2002 ON or NUN 02002 ON or NUN2002 ONS). The invention further relates to vegetative reproductions ofNUN 2002 ON methods for in vitro tissue culture of NUN 2002 ON explantsand also to phenotypic variants of NUN 2002 ON. The invention furtherrelates to methods of producing bulbs or bulblets of NUN 2002 ON.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include greateryield, resistance to insects or pests, tolerance to heat and drought,desired earliness, better agronomic quality, higher nutritional value,growth rate and bulb properties.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different varieties produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines are developed byselfing and selection of desired phenotypes. The new lines are evaluatedto determine which of those have commercial potential.

Onions belong to the lily family, Amaryllidaceae, and the genus Allium.Alliums comprise a group of perennial herbs having bulbous,onion-scented underground leaves, commonly known as onion bulbs,including such commonly cultivated crops as garlic, chives, andshallots. It also includes ornamental species grown for their flowers.

Onions are an important vegetable world-wide, ranking second among allvegetables in economic importance with an estimated production of over80 million tons in 2012 (FAOSTAT). The onion is also one of the oldestcultivated vegetables in history. In general, the bulb is harvested andeaten. The common garden onions belong to the species Allium cepa.Onions are classified in numerous ways, by basic use, flavor, color,shape of the bulb, and day length. Onions come in white, yellow, and redcolors. The bulb may be rounded, flattened, or tapering cylindrical.

Commercial onions include “storage onions”, “fresh onions”, “pearl ormini onions”, and “green onions”. “Fresh onions” tend to have a lightercolor with a thin skin, a milder, sweeter flavor, and must be eatenfresh as they do not store well. These onions are available in red,yellow, and white colors.

Storage onions are available from harvest, which is at the beginning ofAugust, and are stored and available throughout the winter months up toabout March. Storage onions have a darker skin that is thicker than thatof a fresh onion. They are also known for intense, pungent flavor,higher percentage of solids and desirable cooking characteristics. Theseonions are also available in red, yellow and white colors. Not all longday length type (long day type) onions are suitable for storage. A truestorage onion is one that can be harvested in late summer or fall, andstored, under proper conditions, until the spring, when the fresh onioncrop is again available.

“Spanish onion”, “Spanish onions”, or “Spanish type” are terms appliedto various long-day onions, generally yellow, though some white, andgenerally varieties that are large and globe-shaped. Spanish onion iscommonly applied to various long day type onions of the type grown inwestern states of the United States (California, Idaho, Oregon,Washington, Colorado) with a bulb size averaging 300-700 grams (g)(typically over 3 inches up to 4 inches but also up to 5 inches indiameter for bulbs classified as “colossal”).

Onion varieties initiate bulbing when both the temperature and a minimumnumber of daylight hours reach certain levels. When onions are firstplanted, they initially develop their vegetative growth, with no sign ofbulb formation until the proper day length for that onion varietytriggers the signal to the plant to stop producing above groundvegetative growth and start forming a bulb. Onions are thus sensitive tothe hours of daylight and darkness they receive, and for most varietiesit is only when the specific combination of daylight and darkness isreached, that the bulb starts to form. Onions are therefore classifiedby the degree of day length that will initiate bulb formation. Onionsare described as short-, intermediate-, and long-day length types. Shortday means that bulbing will initiate at 11 to 12 hours of daylight.Intermediate day is used for onions bulbing at 12 to about 14 hours ofdaylight. Long day onions require about 14 or more hours of daylight forbulb formation to start.

Growers producing onions in more northerly climates plant long-daylength onions. Daylight length varies greatly with latitude, and athigher latitudes long-day onions will produce sufficient top growthbefore the day length triggers bulbing to produce a large bulb. Ashort-day onion grown in the North (higher latitudes) will bulb tooearly and produce relatively small bulbs.

Short day onions are preferred for southern areas such as southernTexas, southern California and Mexico. If a long day type onion isplanted in such a short day climate, it may never experience enough daylength to trigger the bulbing process.

Onions are also classified on flavor, with the common designations ofsweet, mild, and pungent. The flavor of the onion is a result of boththe type of onion and the growing conditions. For instance, soilscontaining a high amount of sulfur result in more pungent flavoredonions. Sweetness in onions is caused by the sugars glucose, fructoseand sucrose. Onions also contain polymers of fructose called fructans.Onion cultivars differ quite markedly in the relative amounts ofsucrose, glucose, fructose and fructans which they contain. They alsodiffer in sugars according to length of storage and location in thebulb. Short day cultivars, which are poor storers, tend to have higherlevels of sucrose, fructose and glucose, but hardly any of the fructans.In contrast, long day type cultivars and intermediate storage cultivarssuch as Pukekohe Longkeeper have less sucrose, glucose and fructose andhigher amounts of fructans.

Short day varieties do not keep well in storage conditions, and thepungency of short day varieties can climb considerably during storage.Present production in North America and Europe allows harvest of shortday onions from mild winter regions from November through April. Longday onions are available fresh in the late summer and as storage onionsfrom September through March, or even year round, have not beenavailable in low pungency varieties (with the exception of U.S. patentapplication Ser. No. 12/861,740 which is based on patent applicationSer. No. 12/020,360). Sweet onions must be imported from the southernhemisphere to fill the gap in sweet onion production(November-February). In the United States, regions like Georgia andTexas produce short day onions from March to June, while low pungencyonions available from November to February are short day onions,produced in the southern hemisphere.

The use of a type of onion is depending on a customer's preference fortaste, aroma, appearance and color of an onion. There is thus a need fornew short day onions with new appearance and color properties.

SUMMARY OF THE INVENTION

In one aspect of the invention, a seed of onion variety NUN 2002 ON isprovided, wherein a representative sample of said seed has beendeposited under Accession Number NCIMB 42694. The onion seed of theinvention may be provided as an essentially homogeneous population ofonion seed. Therefore, seed of the invention may be defined as formingat least about 97% of the total seed, including at least about 98%, 99%or more of the seed. The population of onion seed may be particularlydefined as being essentially free from non-hybrid seed. The seedpopulation may be separately grown to provide an essentially homogeneouspopulation of onion plants according to the invention. Also encompassedare plants grown from seeds of onion variety NUN 2002 ON and plant partsthereof such as a leaf, pollen, an ovule, a bulb and a cell. Theinvention also provides for a plurality of seeds of the new variety,plants produced from growing the seeds of the new variety NUN 2002 ON,and progeny of any of these.

The invention also concerns plants of onion variety NUN 2002 ON. Theinvention also provides for a plurality of seeds of the new variety,plants produced from growing the seeds of the new variety NUN 2002 ON,and progeny of any of these. Another aspect refers to an onion plant, ora part thereof, having all or all but 1, 2, 3, 4, or 5 the physiologicaland morphological characteristics of an onion plant of onion variety NUN2002 ON.

In one aspect, such progeny have all the physiological and morphologicalcharacteristics of onion variety NUN 2002 ON when grown under the sameenvironmental conditions. In another aspect such progeny have all thephysiological and morphological characteristics as listed in Table 1 asonion variety NUN 2002 ON when measured under the same environmentalconditions (i.e. evaluated at significance levels of 1%, 5% or 10%significance, which can also be expressed as a p value).

In another aspect a plant of the invention or said progeny plantshas/have 1, 2, 3, 4 or more or all of the distinguishing characteristicsselected from the group consisting of: 1) average bulb height of about10.84 cm e.g. 10.2, 10.4, 10.6, 10.8, 11.0, 11.2, 11.4, or 11.6 cm; 2)average bulb diameter of about 10.21 cm e.g. 9.8, 10.0, 10.2, 10.4,10.6, 10.8, 11.0 or 11.2 cm; 3) average bulb weight of about 475 grame.g. 430, 440, 450, 460, 470, 480, 490, 500, 510 or 520 gram; 4) averagecolumn length of sheath (height from soil line to base of lowestsucculent leaf) of about 28 mm e. g. 20, 22, 24, 26, 28, 30, 32, 34, 36or 38 mm; 5) average sheath diameter (at mid-length) of about 20.2 mm e.g. 18, 19, 20, 21, 22, 23 or 24 mm; 6) an adaptation range between 10 to30 degrees mean latitude; 7) a maximum number of inflorescences perplant of two; 8) an average number of inflorescences per plant of one;and 9) compact inflorescence (umbel). In another aspect a plant of theinvention has in addition to the 1, 2, 3, 4 or more or all of theabove-cited distinguishing characteristics, 3, 4, 5, 6, 7, 8, or more,or all of the other (average) characteristics as listed in Table 1.

Further, an onion bulb produced on a plant grown from these seeds isprovided.

In yet another embodiment of the invention, a plant having one, two orthree physiological and/or morphological characteristics which aredifferent from those of NUN 2002 ON and which otherwise has all thephysiological and morphological characteristics of NUN 2002 ON as listedin Table 1, wherein a representative sample of seed of variety NUN 2002ON has been deposited under Accession Number NCIMB 42694, is provided.

Further, a vegetatively propagated plant of variety NUN 2002 ON, or apart thereof, is provided having all the morphological and physiologicalcharacteristics of NUN 2002 ON when grown under the same environmentalconditions.

Also a plant part derived from variety NUN 2002 ON is provided, whereinsaid plant part is selected from the group consisting of: bulbs,harvested bulbs, parts of bulbs, scales, parts of scales, bulblets,parts of bulblets, leaves, parts of leaves, pollen, ovule, cells,petioles, fruits, shoots or parts thereof, stems or parts thereof, rootsor parts thereof, cuttings, seeds, hypocotyl, cotyledon, flowers orparts thereof, and flower. Bulbs are particularly important plant parts.In yet another aspect, seeds of NUN 2002ON are provided. In stillanother aspect, seeds growing on plants of NUN 2002 ON are provided.

In another aspect of the invention, a tissue culture of regenerablecells of a plant of variety NUN 2002 ON is provided. The tissue culturewill preferably be capable of regenerating plants capable of expressingall of the physiological and morphological characteristics of a plant ofthe invention, and of regenerating plants having substantially the samegenotype as other such plants. Examples of some such physiological andmorphological characteristics include those traits set forth in Table 1herein. The regenerable cells in such tissue cultures may be derived,for example, from embryos, meristems, cotyledons, pollen, leaves,anthers, roots, root tips, pistil, flower, seed and stalk. Thus, atissue culture may comprise regenerable cells from embryos, meristems,cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower,seed and bulbs. Still further, the present invention provides onionplants regenerated from a tissue culture of the invention, the plantshaving all the physiological and morphological characteristics of aplant of the invention.

The invention also concerns methods for vegetative propagating of aplant of the invention. In certain embodiments, the method comprises thesteps of: (a) collecting tissue capable of being propagated from a plantof the invention; (b) cultivating said tissue to obtain proliferatedshoots; and (c) rooting said proliferated shoots to obtain rootedplantlets. In some of these embodiments, the method further comprisesgrowing plants from said rooted plantlets.

In yet another aspect of the invention, processes are provided forproducing onion seeds, plants and bulbs, which processes generallycomprise crossing a first parent onion plant with a second parent onionplant, wherein at least one of the first or second parent onion plantsis a plant of the variety designated NUN 2002 ON.

These processes may be further exemplified as processes for preparinghybrid onion seed or plants, wherein a first onion plant is crossed witha second onion plant of a different, distinct variety to provide ahybrid that has, as one of its parents, the onion plant variety NUN 2002ON.

In another embodiment of the invention, onion variety NUN 2002 ON iscrossed to produce onion seed derived of the variety designated NUN 2002ON. In any cross herein, either parent may be the male or female parent.In these processes, crossing will result in the production of seed. Theseed production occurs regardless of whether the seed is collected ornot.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and a second parent onion plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of the firstand the second parent onion plants into plants that bear flowers. Athird step may comprise preventing self-pollination of the plants, suchas by emasculating the male portions of flowers, (e.g., treating ormanipulating the flowers to produce an emasculated parent onion plant).Self-incompatibility systems may also be used in some hybrid crops forthe same purpose. Self-incompatible plants still shed viable pollen andcan pollinate plants of other varieties but are incapable of pollinatingthemselves or other plants of the same variety.

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent onion plants. In certain embodiments, pollenmay be transferred manually or by the use of insect vectors. Yet anotherstep comprises harvesting the seeds from at least one of the parentonion plants. The harvested seed can be grown to produce an onion plantor hybrid onion plant.

The present invention also provides the onion seeds and plants producedby a process that comprises crossing a first parent onion plant with asecond parent onion plant, wherein at least one of the first or secondparent onion plants is a plant provided herein, such as from variety NUN2002 ON. In another embodiment of the invention, onion seed and plantsproduced by the process are first filial generation (F1) hybrid onionseed and plants produced by crossing a plant in accordance with theinvention with another, distinct plant. The present invention furthercontemplates plant parts of such an F1 hybrid onion plant, and methodsof use thereof. Therefore, certain exemplary embodiments of theinvention provide an F1 hybrid onion plant and seed thereof.

In still yet another aspect, the present invention provides a method ofproducing a plant or a seed derived from variety NUN 2002 ON, the methodcomprising the steps of: (a) preparing a progeny plant derived from saidvariety by crossing a plant of variety NUN 2002 ON with a second plant;and (b) selfing the progeny plant or crossing it to the second plant orto a third plant to produce a seed of a progeny plant of a subsequentgeneration.

The method may additionally comprise: (c) growing a progeny plant of afurther subsequent generation from said seed of a progeny plant of asubsequent generation and selfing the progeny plant of a subsequentgeneration or crossing it to the second, the third, or a further plant;and repeating the steps for 3 or more times, e.g., an additional 3-10generations to produce a further plant derived from the aforementionedstarting variety. The further plant derived from variety NUN 2002 ON maybe an inbred variety, and the aforementioned repeated crossing steps maybe defined as comprising sufficient inbreeding to produce the inbredvariety. In the method, it may be desirable to select particular plantsresulting from step (c) for continued crossing according to steps (b)and (c). By selecting plants having one or more desirable traits, aplant is obtained which possesses some of the desirable traits of thestarting plant as well as potentially other selected traits.

One aspect of the invention refers to a method of producing an onionplant comprising crossing an onion plant of variety NUN 2002 ON with asecond onion plant one or more times. This method comprises in oneembodiment selecting progeny from said crossing.

For example, in certain embodiments, the invention provides methods ofintroducing a desired trait into a plant of the invention comprising:(a) crossing a plant of variety NUN 2002 ON with a second onion plantthat comprises a desired trait to produce F1 progeny, (b) selecting anF1 progeny that comprises the desired trait(s), e.g., one, two, three ormore desired trait(s), (c) crossing the selected F1 progeny with a plantof variety NUN 2002 ON to produce backcross progeny, and (d) selectingbackcross progeny comprising the desired trait(s) and which otherwisehas all the physiological and morphological characteristics of varietyNUN 2002 ON. Optionally, steps (c) and (d) can be repeated one or moretimes, e.g., three or more times such as three, four, five, six or seventimes, in succession to produce selected fourth, fifth, sixth, seventhor eighth or higher backcross progeny that comprises the desired trait.The invention also provides onion plants produced by these methods.

In still yet another aspect, the invention provides a method ofdetermining the genotype of a plant of the invention comprisingdetecting in the genome of the plant at least a first polymorphism. Themethod may, in certain embodiments, comprise detecting a plurality ofpolymorphisms in the genome of the plant, for example by obtaining asample of nucleic acid from a plant and detecting in said nucleic acidsa plurality of polymorphisms. The method may further comprise storingthe results of the step of detecting the plurality of polymorphisms on acomputer readable medium.

In one embodiment of the invention, the invention provides a method forproducing a seed of a variety derived from NUN 2002 ON comprising thesteps of

-   (a) crossing an onion plant of variety NUN 2002 ON with a second    onion plant; and-   (b) allowing seed of an onion plant derived from variety NUN 2002 ON    to form. This method can further comprise steps of-   (c) crossing a plant grown from said variety NUN 2002 ON-derived    onion seed with itself or a second onion plant to yield additional    variety NUN 2002 O-derived onion seed;-   (d) growing said additional variety NUN 2002 ON-derived onion seed    of step (c) to yield additional variety NUN 2002 ON-derived onion    plants; and optionally-   (e) repeating the crossing and growing steps of (c) and (d) to    generate further variety NUN 2002 ON-derived onion plants. For    example, the second onion plant is of an inbred onion variety.

In certain embodiments, the present invention provides a method ofproducing onions comprising: (a) obtaining a plant of the invention,wherein the plant has been cultivated to maturity, and (b) collecting anonion bulb from said plant.

The invention also provides for a food or feed product comprising orconsisting of a plant part described herein preferably an onion bulb orpart thereof and/or an extract from a plant part described herein. Thefood or feed product may be fresh or processed, e.g., canned, steamed,boiled, fried, blanched and/or frozen, etc.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DEFINITIONS

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

The term “about” in relation to a particular value refers to saidvalue+/−5%, i.e. to a range between said value minus 5% of said valueand said value plus 5% of said value.

“Onion plant” or “onion” is a plant of genus Allium or a part thereof(e.g. a bulb). Onion includes, e.g., Allium aggregatum (e.g., chalottesand potato onion), Allium cepa and Allium fistulosum, as well ascrossbreds thereof, and hybrids such as Allium×proliferum,Allium×wakegi, and the triploid onion Allium×cornutum.

“Biennial plant” means that Allium cepa L. produces a bulb in the firstseason and seeds in the second.

“Cultivated onion” refers to plants of Allium, i.e. varieties, breedinglines or cultivars of the species Allium cepa as well as crossbreds withAllium aggregatum and Allium fistulosum, cultivated by humans and havinggood agronomic characteristics; preferably such plants are not “wildplants”, i.e. plants which generally have much poorer yields and pooreragronomic characteristics than cultivated plants and e.g. grow naturallyin wild populations. “Wild plants” include for example ecotypes, PI(Plant Introduction) lines, landraces or wild accessions or wildrelatives of Allium and related species.

The terms “Onion plant designated NUN 2002 ON”, “NUN 02002 ON”, “2002ON” or “variety designated NUN 2002 ON” are used interchangeably hereinand refer to an onion plant of variety NUN 2002 ON, representative seedof which having been deposited under Accession Number NCIMB 42694.

“Tissue culture” refers to a composition comprising isolated cells ofthe same or a different type or a collection of such cells organizedinto parts of a plant. Tissue culture of various tissues of onion andregeneration of plants therefrom is well known and widely published(see, e.g., Dunstan and Short (1977) Physiol, Plant, 41: 70-72.; Pikeand Yoo, Scientia Horticulturae, 45 (1990) 31-36. Similarly, the skilledperson is well-aware how to prepare a “cell culture”.

“USDA descriptors” are the plant variety descriptors for onion aspublished by U.S. Department of Agriculture, Agricultural MarketingService, Science and Technology, Plant Variety Protection Office,Beltsville, Md. 20705 (available on the world wide web at ams.usda.gov)in the “Objective description of Variety Onion Allium cepa L.”,ST-470-16 and which can be downloaded from the world wide web atams.usda.gov/AMSv1.0/getfile?dDocName=STELDEV3003776.

“UPOV descriptors” are the plant variety descriptors for onion describedin the “Guidelines for the Conduct of Tests for Distinctness, Uniformityand Stability, TG/46/7 (Geneva 2009), as published by UPOV(International Union for the Protection of New Varieties and Plants,available on the world wide web at upov.int) and which can be downloadedfrom the world wide web at upov.int under edocs/tgdocs/en/tg046.pdf andis herein incorporated by reference in its entirety.

“RHS” refers to the Royal Horticultural Society of England whichpublishes an official botanical color chart quantitatively identifyingcolors according to a defined numbering system, The chart may bepurchased from Royal Horticulture Society Enterprise Ltd RHS Garden;Wisley, Woking; Surrey GU236QB, UK, e.g., the RHS color chart: 2007 (TheRoyal Horticultural Society, PO Box 313 London SW1P2PE).

“Genotype” refers to the genetic composition of a cell or organism.

“Phenotype” refers to the detectable characteristics of a cell ororganism, which characteristics are the manifestation of geneexpression.

As used herein, the term “plant” includes the whole plant or any partsor derivatives thereof, preferably having the same genetic makeup as theplant from which it is obtained, such as plant organs (e.g. harvested ornon-harvested onion bulbs (tubers), leaves etc.), plant cells, plantprotoplasts, plant cell and/or tissue cultures from which whole plantscan be regenerated, plant calli, plant cell clumps, plant transplants,seedlings, hypocotyl, cotyledon, plant cells that are intact in plants,plant clones or micropropagations, or parts of plants (e.g. harvestedtissues or organs), such as plant cuttings, vegetative propagations,embryos, pollen, ovules, flowers, leaves, seeds, clonally propagatedplants, roots, stems, root tips, grafts, parts of any of these and thelike. Also any developmental stage is included, such as seedlings,cuttings prior or after rooting, mature plants or leaves.

By “bulb” or “onion bulb” is meant the (commercially) (harvested orharvestable) edible portion of the onion plant. An onion bulb comprisesan apex and concentric, enlarged fleshy leaf bases, also called fleshyscale leaves. Onion bulbs may be developing onion bulbs or mature onionbulbs.

“Harvested plant material” refers herein to plant parts which have beencollected for further storage and/or further use.

“Harvested seeds” refers to seeds harvested from a line or variety, e.g.produced after self-fertilization or cross-fertilization and collected.

“Flavor” refers to the sensory impression of a food or other substance,especially onion bulb or bulb part and is determined mainly by thechemical senses of taste and smell. Flavor is influenced by textureproperties and by volatile and/or non-volatile chemical components(organic acids, lipids, carbohydrates, etc.). Pungency and sweetness arenon-limiting examples of flavor components of an onion bulb.

“Planting” or “planted” refers to seeding (direct sowing) ortransplanting seedlings (plantlets) into a field by machine or hand.

A plant having “all the physiological and morphological characteristics”of a referred-to-plant means a plant having the physiological andmorphological characteristics of the referred-to-plant when grown underthe same environmental conditions; the referred-to-plant can be a plantfrom which it was derived, e.g. the progenitor plant, the parent, therecurrent parent, the plant used for tissue- or cell culture, etc.

A plant having “essentially all the physiological and morphologicalcharacteristics” of a referred-to-plant means a plant having at least 5(e.g. 6, 7, 8, 9 or all) of the distinguishing physiological andmorphological characteristics (distinguishing characteristics as hereindefined) when grown under the same environmental conditions of thereferred-to-plant (e.g. a plant from which it was derived such as theprogenitor plant, the parent, the recurrent parent, the plant used fortissue- or cell culture, etc.). Alternatively, a plant having“essentially all the physiological and morphological characteristics” ofa referred-to-plant means a plant having all the characteristics aslisted in Table 1 when grown under the same environmental conditions asa referred-to-plant (e.g. a plant from which it was derived such as theprogenitor plant, the parent, the recurrent parent, the plant used fortissue- or cell culture, etc.). In another embodiment, a plant having“essentially all the physiological and morphological characteristics” ofa referred-to-plant means a plant having all but 1, 2, 3, 4 or 5 of thecharacteristics as listed in Table 1 when grown under the sameenvironmental conditions as a referred-to-plant (e.g. a plant from whichit was derived such as the progenitor plant, the parent, the recurrentparent, the plant used for tissue- or cell culture, etc.).

For NUN 2002 ON the distinguishing characteristics are 1) average bulbheight in cm; 2) average bulb diameter in cm; 3) average bulb weight ingram; 4) average column length of sheath (height from soil line to baseof lowest succulent leaf) in mm; 5) average sheath diameter (atmid-length) in mm; 6) adaptation range in degrees mean latitude; 7)maximum number of inflorescences per plant; 8) average number ofinflorescences per plant; and 9) compactness of inflorescence type(umbel).

In certain embodiments the plant of the invention has all thephysiological and morphological characteristics, except for certaincharacteristics mentioned, e.g. the characteristic(s) derived from aconverted or introduced gene or trait and/or except for thecharacteristics which differ.

Similarity between different plants is defined as the number ofdistinguishing characteristics (or the characteristics as listed inTable 1) that are the same between the two plants that are compared whengrown under the same environmental conditions. Characteristics areconsidered “the same” when the value for a numeric characteristic isevaluated at significance levels of 1%, 5% or 10% significance level, orwhen a non-numeric characteristic is identical, if the plants are grownunder the same conditions.

A plant having one or more “essential physiological and/or morphologicalcharacteristics” or one or more “distinguishing characteristics” refersto a plant having (or retaining) one or more of the characteristicsmentioned in Table 1 when grown under the same environmental conditionsthat distinguish NUN 2002 ON from the most similar varieties (such asvariety CARTA BLANCA), such as but not limited to average bulb size,bulb flavor, color and texture, maturity, umbel diameter or average leaflength.

“Distinguishing characteristics” or “distinguishing morphological and/orphysiological characteristics” refers herein the characteristics whichare distinguishing between NUN 2002 ON and other onion varieties, suchas variety CARTA BLANCA, when grown under the same environmentalconditions, especially the following characteristics: 1) average bulbheight of about 10.84 cm e.g. 10.3, 10.4, 10.5. 10.6, 10.7, 10.8, 10.9,11.0, 11.1, 11.2, 11.3; 2) average bulb diameter of about 10.21 cm e.g.9.8, 9.9, 10.0, 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, or 10.8, cm; 3)average bulb weight of about 475 gram e.g. 430, 440, 450, 460, 470, 480,490, 500, 510 or 520 gram; 4) average column length of sheath (heightfrom soil line to base of lowest succulent leaf) of about 28 mm e. g.20, 22, 24, 26, 28, 30, 32, 34, 36 or 38 mm; 5) average sheath diameter(at mid-length) of about 20.2 mm e. g. 18, 19, 20, 21, 22, 23 or 24 mm;6) an adaptation range between 10 to 30 degrees mean latitude; 7) amaximum number of inflorescences per plant of 2; 8) an average number ofinflorescences per plant of 1; and 9) compact inflorescence (umbel). Inone aspect, the distinguishing characteristics further include at leastone, two, three or more (or all) of the characteristics listed in Table1.

Thus, a onion plant “comprising the distinguishing characteristics ofNUN 2002 ON” refers herein to a onion r plant which does not differsignificantly from NUN 2002 ON in characteristics 1) to 5) above. In afurther aspect the onion plant further does not differ significantlyfrom NUN 2002 ON in one or more, or all characteristics 6) to 9) asmentioned above. In yet a further aspect the onion plant further doesnot differ in at least one, two, three, four, five or sixcharacteristics selected from the characteristics listed in Table 1. Instill another aspect the onion plant does not differ in any of thedistinguishing characteristics 1) to 9) listed above.

The physiological and/or morphological characteristics mentioned aboveare commonly evaluated at significance levels of 1%, 5% or 10% % if theyare numerical, or for identical type if not numerical, when measuredunder the same environmental conditions. For example, a progeny plant ofNUN 53016 CUP may have one or more (or all) of the essentialphysiological and/or morphological characteristics of NUN 53016 CUPlisted in Table 1, as determined at the 5% significance level (i.e.p<0.05) when grown under the same environmental conditions.

“Maturity” refers to the development stage of an onion bulb when saidonion bulb has fully developed (reached its final size). In particularembodiments “maturity” is defined as the mature state of bulbdevelopment and optimal time for harvest. Typically, maturity of a bulbis reached when the vegetative phase of an onion plant is over andleaves and neck of the onion plant dry out.

“Harvest maturity” is referred to as the stage at which an onion bulb isready for harvest or the optimal time to harvest the bulb. In oneembodiment, harvest maturity is the stage where 25-50% of the onion leaftops have fallen over.

“Yield” means the total weight of all onion bulbs harvested per surfaceunit or per plant of a particular line or variety. It is understood that“yield” expressed as weight of all onion bulbs harvested per hectare canbe obtained by multiplying the number of plants per hectare times the“yield per plant”.

“Marketable yield” means the total weight of all marketable onion bulbharvested per hectare of a particular line or variety, i.e. bulbssuitable for being sold for fresh consumption, having acceptable shape,moisture, pungency etc., and no or very low levels of deficiencies.

As used herein, a “mature onion bulb” refers to any onion bulb that isready for harvest. Generally, when 25-50% of the onion leaf tops havefallen over, the onion is ready for harvest.

As used herein, the term “variety” or “cultivar” means a plant groupingwithin a single botanical taxon of the lowest known rank, whichgrouping, irrespective of whether the conditions for the grant of abreeder's right are fully met, can be defined by the expression of thecharacteristics resulting from a given genotype or combination ofgenotypes, distinguished from any other plant grouping by the expressionof at least one of the said characteristics and considered as a unitwith regard to its suitability for being propagated unchanged.

“Plant line” is for example a breeding line which can be used to developone or more varieties.

“Hybrid variety” or “F1 hybrid” refers to the seeds of the firstgeneration progeny of the cross of two non-isogenic plants. For example,the female parent is pollinated with pollen of the male parent toproduce hybrid (F1) seeds on the female parent.

“Regeneration” refers to the development of a plant from cell culture ortissue culture or vegetative propagation.

“Vegetative propagation”, “vegetative reproduction” or “clonalpropagation” are used interchangeably herein and mean the method oftaking part of a plant and allowing that plant part to form at leastroots where plant part is, e.g., defined as or derived from (e.g. bycutting of) a bulb or part thereof, leaf, pollen, embryo, cotyledon,hypocotyl, cells, protoplasts, meristematic cell, root, root tip,pistil, anther, flower, shoot tip, shoot, stem, petiole, etc. When awhole plant is regenerated by vegetative propagation, it is alsoreferred to as a vegetative propagation.

The terms “gene converted” or “conversion plant” in this context referto onion plants which are often developed by backcrossing whereinessentially all of the desired morphological and physiologicalcharacteristics of parent are recovered in addition to the one or moregenes transferred into the parent via the backcrossing technique or viagenetic engineering. Likewise a “Single Locus Converted (Conversion)Plant” refers to plants which are developed by plant breeding techniquescomprising or consisting of backcrossing, wherein essentially all of thedesired morphological and physiological characteristics of an onionvariety are recovered in addition to the characteristics of the singlelocus having been transferred into the variety via the backcrossingtechnique and/or by genetic transformation.

“Progeny” as used herein refers to plants derived from a plantdesignated NUN 2002 ON. Progeny may be derived by regeneration of cellculture or tissue culture or parts of a plant designated NUN 2002 ON orselfing of a plant designated NUN 2002 ON or by producing seeds of aplant designated NUN 2002 ON. In further embodiments, progeny may alsoencompass plants derived from crossing of at least one plant designatedNUN 2002 ON with another onion plant of the same or another variety or(breeding) line, or with a wild onion plant, backcrossing, inserting ofa locus into a plant or selecting a plant comprising a mutation. Aprogeny is, e.g., a first generation progeny, i.e. the progeny isdirectly derived from, obtained from, obtainable from or derivable fromthe parent plant by, e.g., traditional breeding methods (selfing and/orcrossing) or regeneration. However, the term “progeny” generallyencompasses further generations such as second, third, fourth, fifth,sixth, seventh or more generations, i.e., generations of plants whichare derived from, obtained from, obtainable from or derivable from theformer generation by, e.g., traditional breeding methods, regenerationor genetic transformation techniques. For example, a second generationprogeny can be produced from a first generation progeny by any of themethods mentioned above.

The term “traditional breeding techniques” encompasses herein crossing,selfing, selection, double haploid production, embryo rescue, protoplastfusion, marker assisted selection, mutation breeding, tissue propagationetc. as known to the breeder (i.e. methods other than geneticmodification/transformation/transgenic methods), by which, for example,a genetically heritable trait can be transferred from one onion line orvariety to another.

“Crossing” refers to the mating of two parent plants.

“Cross-pollination” refers to the fertilization by the union of twogametes from different plants.

“Backcrossing” is a traditional breeding technique used to introduce atrait into a plant line or variety. The plant containing the trait iscalled the donor plant and the plant into which the trait is transferredis called the recurrent parent. An initial cross is made between thedonor parent and the recurrent parent to produce progeny plants. Progenyplants which have the trait are then crossed to the recurrent parent.After several generations of backcrossing and/or selfing the recurrentparent comprises the trait of the donor. The plant generated in this waymay be referred to as a “single trait converted plant”. Onion varietiescan also be developed from more than two parents. The technique, knownas modified backcrossing, uses different recurrent parents during thebackcrossing. Modified backcrossing may be used to replace the originalrecurrent parent with a variety having certain more desirablecharacteristics or multiple parents may be used to obtain differentdesirable characteristics from each.

“Selfing” refers to self-pollination of a plant, i.e., the transfer ofpollen from the anther to the stigma of the same plant.

“Locus” (plural loci) refers to the specific location of a gene or DNAsequence on a chromosome. A locus may confer a specific trait.

“Marker” refers to a readily detectable phenotype, preferably inheritedin co-dominant fashion (both alleles at a locus in a diploidheterozygote are readily detectable), with no environmental variancecomponent, i.e., a heritability of one (1).

“Allele” refers to one or more alternative forms of a gene locus. All ofthese loci relate to one trait. Sometimes, different alleles can resultin different observable phenotypic traits, such as differentpigmentation. However, many variations at the genetic level result inlittle or no observable variation. If a multicellular organism has twosets of chromosomes, i.e. diploid, these chromosomes are referred to ashomologous chromosomes. Diploid organisms have one copy of each gene(and therefore one allele) on each chromosome. If both alleles are thesame, they are homozygotes. If the alleles are different, they areheterozygotes.

As used herein, the terms “resistance” and “tolerance” are usedinterchangeably to describe plants that show no symptoms to a specifiedbiotic pest, pathogen, abiotic influence or environmental condition.These terms are also used to describe plants showing some symptoms butthat are still able to produce marketable product with an acceptableyield. Some plants that are referred to as resistant or tolerant areonly so in the sense that they may still produce a crop, even though theplants are stunted and the yield is reduced.

“Transgene” or “chimeric gene” refers to a genetic locus comprising aDNA sequence which has been introduced into the genome of an onion plantby transformation. A plant comprising a transgene stably integrated intoits genome is referred to as “transgenic plant”.

“Average” refers herein to the arithmetic mean.

The term “mean” refers to the arithmetic mean of several measurements.The skilled person understands that the appearance of a plant depends tosome extent on the growing conditions of said plant. Thus, the skilledperson will know typical growing conditions for onion described herein.The mean, if not indicated otherwise within this application, refers tothe arithmetic mean of measurements on at least 10 different, randomlyselected plants of a variety or line.

“Substantially equivalent” refers to a characteristic that, whencompared, does not show a statistically significant difference (e.g.,p=0.05) from the mean.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to a Allium cepa variety, referred to asNUN 2002 ON, which has a higher bulb length (than check variety CARTABLANCA), a higher bulb diameter (than check variety CARTA BLANCA), ahigher bulb weight (than check variety CARTA BLANCA), a shorter sheathlength (than check variety CARTA BLANCA), a higher sheath diameter (thancheck variety CARTA BLANCA), an adaptation range smaller (than checkvariety CARTA BLANCA), a lower maximum number of inflorescences perplant (than check variety CARTA BLANCA), a lower average number ofinflorescences per plant (than check variety CARTA BLANCA)), compactinflorescence (whereas check variety CARTA BLANCA has loose/openinflorescence). Also encompassed by the present invention are progeny ofNUN 2002 ON and methods of producing plants in accordance with thepresent invention.

An onion plant of NUN 2002 ON differs from the most similar comparisonvariety CARTA BLANCA in one or more characteristics (referred herein toas “distinguishing characteristics” or “distinguishing morphologicaland/or physiological characteristics” (or essential physiological and/ormorphological characteristics) selected from

-   1) NUN 2002 ON has an average bulb height that is at least 4%, 5%,    6%, 7%, or even 7.7% larger than the average bulb height of check    variety CARTA BLANCA;-   2) NUN 2002 ON has an average bulb diameter that is at least 3%, 4%,    5%, 5.5%, or even 6% larger than the average bulb diameter of check    variety CARTA BLANCA;-   3) NUN 2002 ON has an average bulb weight that is at least 7%, 10%,    12%, 13%, or even 13.7% higher than the average bulb weight of check    variety CARTA BLANCA;-   4) NUN 2002 ON has an average column length of sheath (height from    soil line to base of lowest succulent leaf) that is at least 10%,    20%, 25%, 28%, or even 1% lower than the average column length of    sheath of check variety CARTA BLANCA;-   5) NUN 2002 ON has an a average sheath diameter (at mid-length) that    is at least 10%, 14%, 16%, 18%, or even 18.8% higher than the    average sheath diameter of check variety CARTA BLANCA;-   6) NUN 2002 ON has an adaptation range between 10 to 30 degrees mean    latitude, whereas check variety CARTA BLANCA has an adaptation range    between 10 to 35 degrees mean latitude;-   7) NUN 2002 ON has a maximum number of inflorescences per plant of    two, whereas check variety CARTA BLANCA has a maximum number of    inflorescences per plant of three;-   8) NUN 2002 ON has an average number of inflorescences per plant of    one, whereas check variety CARTA BLANCA has an average number of    inflorescences per plant of two;-   9) NUN 2002 ON has compact inflorescences, whereas check variety    CARTA BLANCA has loose/open inflorescences;

It is understood that “significant” differences refer to statisticallysignificant differences, when comparing the characteristic between twoplant lines or varieties when grown under the same conditions.Preferably at least about 10, 15, 20 or more plants per line or varietyare grown under the same conditions (i.e. side by side) andcharacteristics are measured on at least about 10, 15, 20 or morerandomly selected plant or plant parts to obtain averages. Thus,physiological and morphological characteristics or traits are commonlyevaluated at a significance level of 1%, 5% or 10%, when measured inplants grown under the same environmental conditions.

Thus, in one aspect, the invention provides seeds of the onion varietydesignated NUN 2002 ON wherein a representative sample of seeds of saidvariety was deposited under the Budapest Treaty, with Accession numberNCIMB 42694. Seeds of NUN 2002 ON are obtainable by crossing the maleparent with the female parent and harvesting the seeds produced on thefemale parent. The resultant NUN 2002 ON seeds can be grown to produceNUN 2002 ON plants. In one embodiment a plurality of NUN 2002 ON seedsare packaged into small and/or large containers (e.g., bags, cartons,cans, etc.). The seeds may be disinfected, primed and/or treated withvarious compounds, such as seed coatings or crop protection compounds.

Also provided are plants of onion variety NUN 2002 ON, or a bulb orother plant part thereof, produced from seeds, wherein a representativesample of said seeds has been deposited under the Budapest Treaty, withAccession Number NCIMB 42694. Also included is a cell culture or tissueculture produced from such a plant It is understood that such tissue orcell culture comprising cells or protoplasts from the plant of theinvention can be obtained from a plant part selected from the groupconsisting of embryos, meristems, cotyledons, hypocotyl, pollen, leaves,anthers, roots, root tips, bulbs, scales, pistil, petiole, flower,fruit, seed, stem and stalks. In one embodiment a plant regenerated fromsuch a cell or tissue culture said plant expressing all themorphological and physiological characteristics of NUN 2002 ON.

In one embodiment the invention provides a onion plant regenerated fromthe tissue or cell culture of NUN 2002 ON, wherein the plant has all ofthe physiological and morphological characteristics of NUN 2002 ON aslisted in Table 1 when determined at the 5% significance level. Inanother embodiment, the invention provides a onion plant regeneratedfrom the tissue or cell culture of NUN 2002 ON, wherein the plant hasall of the physiological and morphological characteristics of NUN 2002ON when determined at the 5% significance level.

Plants of NUN 2002 ON can be produced by seeding directly in the ground(e.g., field) or by germinating the seeds in controlled environmentconditions (e.g., greenhouses) and then transplanting the seedlings intothe field. For example, the seed can be sown into prepared seed bedswhere they will remain for the entire production of the crop.Alternatively, the onion seed may be planted or transplanted in preparedmounds.

In another aspect, the invention provides for a onion plant of varietyNUN 2002 ON, a representative sample of seed from said variety has beendeposited under the Budapest Treaty, with Accession number NCIMB 42694.

In other aspects, the invention provides for a bulb or parts thereof ofonion variety NUN 2002 ON, or a plant part, such as pollen, flowers,shoots or cuttings of variety NUN 2002 ON or parts thereof.

In one embodiment any plant of the invention comprises at least 3, 4, 5or more, e.g. 6, 7, 8, 9 or all of the following morphological and/orphysiological characteristics (i.e. distinguishing characteristics(average values; measured at harvest or market maturity, as indicated onthe USDA Objective description of variety—Onion (unless indicatedotherwise), when grown under the same environmental conditions):

-   1) NUN 2002 ON has an average bulb height of about 10.84 cm e.g.    between 10.1 and 11.6 cm, or between about 10.4 and 11.3 cm, or    between 10.6 and 11.1 cm, or even between 10.8 and 10.9 cm;-   2) NUN 2002 ON has an average bulb diameter of about 10.21 cm e.g.    between 9.7 and 10.7 cm, or between about 9.8 and 10.6 cm, or    between 10.0 and 10.4 cm, or even between 10.1 and 10.3 cm;-   3) NUN 2002 ON has an average bulb weight of about 475 gram e.g.    between 470 and 480 gram, or between about 460 and 490 gram, or    between 450 and 500 gram, or even between 425 and 525 gram;-   4) NUN 2002 ON has an average column length of sheath (height from    soil line to base of lowest succulent leaf) of about 28 mm e.g.    between 26 and 30 mm, or between about 24 and 32 mm, or between 20    and 34 mm, or even between 18 and 38 mm;-   5) NUN 2002 ON has an average sheath diameter (at mid-length) of    about 20.2 mm e. g. between 19.5 and 20.5 mm, or between 19 and 21    mm, or even between 18 and 22 mm;-   6) NUN 2002 ON has an adaptation range between 10 to 30 degrees mean    latitude, whereas check variety CARTA BLANCA has an adaptation range    between 10 to 35 degrees mean latitude;-   7) NUN 2002 ON has a maximum number of inflorescences per plant of    two, whereas check variety CARTA BLANCA has a maximum number of    inflorescences per plant of three;-   8) NUN 2002 ON has an average number of inflorescences per plant of    one, whereas check variety CARTA BLANCA has an average number of    inflorescences per plant of two;-   9) NUN 2002 ON has compact inflorescences, whereas check variety    CARTA BLANCA has loose/open inflorescences;

In still another aspect the invention provides a method of producing aonion plant, comprising crossing a plant of onion variety NUN 2002 ONwith a second onion plant one or more times, and selecting progeny fromsaid crossing.

In yet another aspect the invention provides a method of producing aonion plant, comprising selfing a plant of onion variety NUN 2002 ON oneor more times, and selecting progeny from said selfing.

In other aspects, the invention provides for progeny of variety NUN 2002ON such as progeny obtained by further breeding NUN 2002 ON. Furtherbreeding NUN 2002 ON includes selfing NUN 2002 ON one or more timesand/or cross-pollinating NUN 2002 ON with another onion plant or varietyone or more times. In particular, the invention provides for progenythat retain all the essential morphological and physiologicalcharacteristics of NUN 2002 ON or that retain one or more (e.g. 1) to 5)or 1) to 9) or all) of the distinguishing characteristics of the oniontype described further above, or, in another embodiment, progeny thatretain all morphological and physiological characteristics of NUN 2002ON as listed in Table 1; when grown under the same environmentalconditions, when determined at the 5% significance level. In anotheraspect, the invention provides for vegetative reproductions of thevariety and plants having all but 1, 2, or 3 of the physiological andmorphological characteristics of NUN 2002 ON (e.g. as listed in Table1).

The morphological and/or physiological differences between plantsaccording to the invention, i.e. NUN 2002 ON or progeny thereof, orplants having all but 1, 2, or 3 of the physiological and morphologicalcharacteristics of NUN 2002 ON (as listed in Table 1); and other knownvarieties can easily be established by growing NUN 2002 ON next to theother varieties (in the same field, under the same environmentalconditions), preferably in several locations which are suitable for saidonion cultivation, and measuring morphological and/or physiologicalcharacteristics of a number of plants (e.g., to calculate an averagevalue and to determine the variation range/uniformity within thevariety). For example, trials can be carried out in Acampo Calif., USA(N 38 degrees 07′261″/W 121 degrees 18′ 807″, USA, whereby maturity,leaf shape, leaf color, flower size and color, bulb type, bulb color,bulb size, bulb shape, onion sweetness and pungency, disease resistance,insect resistance, can be measured and directly compared for species ofAllium cepa.

The morphological and physiological characteristics (and distinguishingcharacteristics) of NUN 2002 ON, are provided in Table 1. Encompassedherein are also plants derivable from NUN 2002 ON (e.g. by selfingsand/or crossing and/or backcrossing with NUN 2002 ON and/or progenythereof) comprising all the physiological and morphologicalcharacteristics of NUN 2002 ON listed in Table 1 as determined at the 5%significance level when grown under the same environmental conditionsand/or comprising one or more (or all; or all except one, two or three)of the distinguishing characteristics as determined at the 5%significance level when grown under the same environmental conditions.

Also at-harvest and/or post-harvest characteristics of bulbs can becompared, such as storage holding quality or scale retention, can bemeasured using known methods.

The morphological and/or physiological characteristics may vary somewhatwith variation in the environment (such as temperature, light intensity,day length, humidity, soil, fertilizer use), which is why a comparisonunder the same environmental conditions is preferred. Colors can best bemeasured against The Munsell Book of Color (Munsell Color MacbethDivision of Kollmorgan Instruments Corporation) or using the RoyalHorticultural Society Chart (World wide web atrhs.org.uk/Plants/RHS-Publications/RHS-colour-charts).

In a preferred embodiment, the invention provides for onion bulbs ofvariety NUN 2002 ON, or a part of the bulb. In another embodiment, theinvention provides for a container comprising or consisting of aplurality of harvested onion bulbs of NUN 2002 ON, or progeny thereof.In yet a further embodiment, the invention provides for a method ofproducing a new onion plant. The method comprises crossing a plant ofthe invention NUN 2002 ON, or a plant comprising all but 1, 2, or 3 ofthe morphological and physiological characteristics of NUN 2002 ON (aslisted in Table 1), or a progeny plant thereof, either as male or asfemale parent, with a second onion plant (or a wild relative of onion)one or more times, and/or selfing a onion plant according to theinvention i.e. NUN 2002 ON, or a progeny plant thereof, one or moretimes, and selecting progeny from said crossing and/or selfing. Thesecond onion plant may for example be a line or variety of the speciesAllium cepa, or other Allium species.

Progeny are a later generation (of seeds) produced from the first crossof the F1 hybrid with another plant (F2) or with itself (S2), or anyfurther generation produced by crossing and/or selfing (F3, F4, etc.)and/or backcrossing (BC2, BC3, etc.) one or more selected plants of theF2 and/or S2 and/or BC2 generation (or plants of any further generation,e.g. the F3) with another onion plant (and/or with a wild relative ofonion). Progeny may have all the physiological and morphologicalcharacteristics of onion variety NUN 2002 ON when grown under the sameenvironmental conditions and/or progeny may have (be selected forhaving) one or more of the distinguishing characteristics of onion ofthe invention. Using common breeding methods such as backcrossing orrecurrent selection, one or more specific characteristics may beintroduced into NUN 2002 ON, to provide or a plant comprising all but 1,2, or 3 or more of the morphological and physiological characteristicsof NUN 2002 ON (as listed in Table 1).

The invention provides for methods of producing plants which retain allthe morphological and physiological characteristics of NUN 2002 ON. Theinvention provides also for methods of producing a plant comprising allbut 1, 2, or 3 or more of the morphological and physiologicalcharacteristics of NUN 2002 ON (e.g. as listed in Table 1), but whichare still genetically closely related to NUN 2002 ON. The relatednesscan, for example be determined by fingerprinting techniques (e.g.,making use of isozyme markers and/or molecular markers such as SNPmarkers, AFLP markers, microsatellites, minisatellites, RAPD markers,RFLP markers and others). A plant is “closely related” to NUN 2002 ON ifits DNA fingerprint is at least 80%, 90%, 95% or 98% identical to thefingerprint of NUN 2002 ON. In a preferred embodiment AFLP markers areused for DNA fingerprinting (Vos et al. 1995, Nucleic Acid Research 23:4407-4414). A closely related plant may have a Jaccard's Similarityindex of at least about 0.8, preferably at least about 0.9, 0.95, 0.98or more (SANTOS, CAF et al. Hortic. Bras. 2011, vol. 29, n.1, pp.32-37.). The invention also provides plants and varieties obtained bythese methods. Plants may be produced by crossing and/or selfing, oralternatively, a plant may simply be identified and selected amongst NUN2002 ON plants, or progeny thereof, e.g. by identifying a variant withinNUN 2002 ON or progeny thereof (e.g. produced by selfing) which variantdiffers from NUN 2002 ON in one, two or three of the morphologicaland/or physiological characteristics (e.g. in one, two or threedistinguishing characteristics), e.g. those listed in Table 1 or others.In one embodiment the invention provides a onion plant having aJaccard's Similarity index with NUN 2002 ON of at least 0.8, e.g. atleast 0.85, 0.9, 0.95, 0.98 or even at least 0.99.

By crossing and/or selfing also (one or more) single traits may beintroduced into the variety of the invention i.e. NUN 2002 ON (e.g.,using backcrossing breeding schemes), while retaining the remainingmorphological and physiological characteristics of NUN 2002 ON and/orwhile retaining one or more distinguishing characteristics. A singletrait converted plant may thereby be produced. For example, diseaseresistance genes may be introduced, genes responsible for one or morequality traits, yield, etc. Both single genes (dominant or recessive)and one or more QTLs (quantitative trait loci) may be transferred intoNUN 2002 ON by breeding with NUN 2002 ON.

Any pest or disease resistance genes may be introduced into a plantaccording to the invention, i.e. NUN 2002 ON, progeny thereof or into aplant comprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of NUN 2002 ON (e.g. as listed in Table1). Resistance to one or more of the following diseases is preferablyintroduced into plants of the invention: Black Mold, Neck Mold, PupleBlotch, Smut, Mildew, Pink root, Smudge, Yellow dwarf and Thrips. Otherresistance genes, against pathogenic viruses, fungi, bacteria,nematodes, insects or other pests may also be introduced.

Thus, invention also provides a method for developing a onion plant in aonion breeding program, using a onion plant of the invention, or itsparts as a source of plant breeding material. Suitable plant breedingtechniques are recurrent selection, backcrossing, pedigree breeding,mass selection, mutation breeding and/or genetic marker enhancedselection. For example, in one aspect, the method comprises crossing NUN2002 ON or progeny thereof, or a plant comprising all but 1, 2, or 3 ormore of the morphological and physiological characteristics of NUN 2002ON (e.g. as listed in Table 1), with a different onion plant, andwherein one or more offspring of the crossing are subject to one or moreplant breeding techniques selected from the group consisting ofrecurrent selection, backcrossing, pedigree breeding, mass selection,mutation breeding and genetic marker enhanced selection (see e.g.McCallum et al., Theor Appl Genet (2006) 112: 958-967). For breedingmethods in general see Principles of Plant Genetics and Breeding, 2007,George Acquaah, Blackwell Publishing, ISBN-13: 978-1-4051-3646-4.

The invention thus also provides a method of introducing a single locusconversion, or single trait conversion or introducing a desired trait,into a onion plant according to the invention and/or into NUN 2002 ONcomprising:

-   (a) crossing a onion plant of variety NUN 2002 ON, a representative    sample of seed of said variety having been deposited under Accession    Number NCIMB 42694, with a second onion plant comprising a desired    single locus to produce F1 progeny plants;-   (b) selecting F1 progeny plants that have the single locus;-   (c) crossing the selected progeny plants with a plant of NUN 2002    ON, to produce backcross progeny plants;-   (d) selecting backcross progeny plants that have the single locus    and one or more (or all) distinguishing characteristics of onion    according to the invention and/or all the physiological and    morphological characteristics of NUN 2002 ON to produce selected    backcross progeny plants; and-   (e) optionally repeating steps (c) and (d) one or more times in    succession to produce selected second, third or fourth or higher    backcross progeny plants that comprise the single locus and    otherwise one or more (or all) the distinguishing characteristics of    the onions according to the invention and/or comprise all of the    physiological and morphological characteristics of NUN 2002 ON, when    grown in the same environmental conditions. The invention further    relates to plants obtained by this method.

The above method is provided, wherein the single locus confers a trait,wherein the trait is pest resistance or disease resistance.

In one embodiment the trait is disease resistance and the resistance isconferred to Black Mold, Neck Mold, Purple Blotch, Smut, Mildew, Pinkroot, Smudge, Yellow dwarf and Thrips.

The invention also provides a onion plant comprising at least a firstset of the chromosomes of onion variety NUN 2002 ON, a sample of seed ofsaid variety having been deposited under Accession Number NCIMB 42694;optionally further comprising a single locus conversion, wherein saidplant has essentially all of the morphological and physiologicalcharacteristics of the plant comprising at least a first set of thechromosomes of onion NUN 2002 ON. In another embodiment, this singlelocus conversion confers a trait selected from the group consisting ofmale sterility, herbicide tolerance, pathogen resistance (e.g., insectresistance, nematode resistance, resistance to bacterial, fungal, andviral disease), environmental stress tolerance, modified carbohydratemetabolism, modified protein metabolism improved harvestcharacteristics, enhanced nutritional quality, increased antioxidantcontent, improved processing characteristics, high yield, improvedcharacteristics related to the bulb flavor, texture, size, shape,durability, shelf life, and yield, increased soluble solids content,uniform ripening, delayed or early ripening, adaptability for soilconditions, and adaptability for climate conditions.

In one embodiment, NUN 2002 ON may also be mutated (by e.g. irradiation,chemical mutagenesis, heat treatment, etc.) and mutated seeds or plantsmay be selected in order to change one or more characteristics of NUN2002 ON. Methods such as TILLING may be applied to onion populations inorder to identify mutants. Similarly, NUN 2002 ON may be transformed andregenerated, whereby one or more chimeric genes are introduced into thevariety or into a plant comprising all but 1, 2, 3, or more of themorphological and physiological characteristics (e.g. as listed in Table1). Transformation can be carried out using standard methods, such asAgrobacterium tumefaciens mediated transformation or biolistics,followed by selection of the transformed cells and regeneration intoplants. A desired trait (e.g. genes conferring pest or diseaseresistance, herbicide, fungicide or insecticide tolerance, etc.) can beintroduced into NUN 2002 ON, or progeny thereof, by transforming NUN2002 ON or progeny thereof with a transgene that confers the desiredtrait, wherein the transformed plant retains all the phenotypic and/ormorphological and/or physiological characteristics of NUN 2002 ON or theprogeny thereof and contains the desired trait.

The invention also provides for progeny of onion hybrid (F1) variety NUN2002 ON obtained by further breeding with NUN 2002 ON. In one aspectprogeny are F2 progeny obtained by crossing NUN 2002 ON with anotherplant or S2 progeny obtained by selfing NUN 2002 ON. Also encompassedare F3 progeny obtained by selfing the F2 plants. “Further breeding”encompasses traditional breeding (e.g., selfing, crossing,backcrossing), marker assisted breeding, and/or mutation breeding. Inone embodiment, the progeny have one or more (or all) of thedistinguishing characteristics mentioned further above when grown underthe same environmental conditions. In a further embodiment the progenyhave all the physiological and morphological characteristics of varietyNUN 2002 ON when grown under the same environmental conditions. Inanother embodiment the progeny have one, two, or three distinct traits(qualitative or quantitative) introduced into NUN 2002 ON, whileretaining all the other physiological and morphological characteristicsof variety NUN 2002 ON when grown under the same environmentalconditions.

The invention also provides a plant having one, two or threephysiological and/or morphological characteristics which are differentfrom those of NUN 2002 ON and which otherwise has all the physiologicaland morphological characteristics of NUN 2002 ON, wherein arepresentative sample of seed of variety NUN 2002 ON has been depositedunder Accession Number NCIMB 42694. In particular plants which differfrom NUN 2002 ON in none, one, two or three of the characteristicsmentioned in Table 1 are encompassed.

In one aspect, the plant having one, two or three physiological and/ormorphological characteristics which are different from those of NUN 2002ON and which otherwise has all the physiological and morphologicalcharacteristics of NUN 2002 ON differs from NUN 2002 ON in one, two orthree of the distinguishing morphological and/or physiologicalcharacteristics selected from 1) average bulb height in cm; 2) averagebulb diameter in cm; 3) average bulb weight in gram; 4) average columnlength of sheath (height from soil line to base of lowest succulentleaf) in mm; 5) average sheath diameter (at mid-length) in mm; 6)adaptation range in degrees mean latitude; 7) maximum number ofinflorescences per plant; 8) average number of inflorescences per plant;and 9) compactness of inflorescence type (umbel).

In another embodiment the plant having one, two or three physiologicaland/or morphological characteristics which are different from those ofNUN 2002 ON and which otherwise has all the physiological andmorphological characteristics of NUN 2002 ON differs from NUN 2002 ON inone, two or three morphological or physiological characteristic otherthan the “distinguishing morphological and/or physiologicalcharacteristics” (or essential physiological and/or morphologicalcharacteristics) of NUN 2002 ON selected from: 1) average bulb height incm; 2) average bulb diameter in cm; 3) average bulb weight in gram; 4)average column length of sheath (height from soil line to base of lowestsucculent leaf) in mm; 5) average sheath diameter (at mid-length) in mm;6) adaptation range in degrees mean latitude; 7) maximum number ofinflorescences per plant; 8) average number of inflorescences per plant;and 9) compactness of inflorescence type (umbel).

Onions according to the invention, such as the variety NUN 2002 ON, orits progeny, or a plant having all physiological and/or morphologicalcharacteristics but one, two or three which are different from those ofNUN 2002 ON, can also be reproduced using vegetative reproductionmethods. Therefore, the invention provides for a method of producingplants, or a part thereof, of variety NUN 2002 ON, comprising vegetativepropagation of variety NUN 2002 ON. Vegetative propagation comprisesregenerating a whole plant from a plant part of variety NUN 2002 ON (orfrom its progeny or from or a plant having all physiological and/ormorphological characteristics but one, two or three, which are differentfrom those of NUN 2002 ON), such as a cutting, a cell culture or atissue culture.

The invention also concerns methods of vegetatively propagating a plantof the invention. In certain embodiments, the method comprises the stepsof: (a) collecting tissue or cells capable of being propagated from aplant of the invention; (b) cultivating said tissue or cells to obtainproliferated shoots; and (c) rooting said proliferated shoots, to obtainrooted plantlets. Steps (b) and (c) may also be reversed, i.e. firstcultivating said tissue to obtain roots and then cultivating the tissueto obtain shoots, thereby obtaining rooted plantlets. The rootedplantlets may then be further grown, to obtain plants. In oneembodiment, the method further comprises step (d) growing plants fromsaid rooted plantlets

The invention also provides for a vegetatively propagated plant ofvariety NUN 2002 ON (or from its progeny or from or a plant having allbut one, two or three physiological and/or morphological characteristicswhich are different from those of NUN 2002 ON, or a part thereof, havingone or more distinguishing characteristics and/or all the morphologicaland physiological characteristics of NUN 2002 ON (except for thecharacteristics differing), when grown under the same environmentalconditions.

Parts of NUN 2002 ON (or of its progeny or of a plant having allphysiological and/or morphological characteristics but one, two or threewhich are different from those of NUN 2002 ON) encompass any cells,tissues, organs obtainable from the seedlings or plants, such as but notlimited to: onion bulbs or parts thereof, cuttings, hypocotyl,cotyledon, pollen, scion and the like. Such parts can be stored and/orprocessed further. Encompassed are therefore also food or feed productscomprising one or more of such parts, such as canned, chopped, cooked,roasted, preserved, frozen, fried, dried, pickled, or pureed onion bulbsfrom NUN 2002 ON or from progeny thereof, such as a plant having all butone, two or three physiological and/or morphological characteristicswhich are different from those of NUN 2002 ON.

In one aspect haploid plants and/or double haploid plants of NUN 2002ON, or a plant having all but one, two or three physiological and/ormorphological characteristics which are different from those of NUN 2002ON, or progeny of any of these, are encompassed herein. Haploid anddouble haploid (DH) plants can, for example, be produced by cell ortissue culture and chromosome doubling agents and regeneration into awhole plant. For DH production chromosome doubling may be induced usingknown methods, such as colchicine treatment or the like.

Also provided are plant parts derived from variety NUN 2002 ON (or fromits progeny or from a plant having all but one, two or threephysiological and/or morphological characteristics which are differentfrom those of NUN 2002 ON D), or from a vegetatively propagated plant ofNUN 2002 ON (or from its progeny or from a plant having all but one, twoor three physiological and/or morphological characteristics which aredifferent from those of NUN 2002 ON), being selected from the groupconsisting of: harvested bulbs or parts thereof, pollen, cells, leavesor parts thereof, petioles, cotyledons, hypocotyls, shoots or partsthereof, stems or parts thereof, or vines or parts thereof, roots orparts thereof, cuttings, or flowers.

In one embodiment, the invention provides for extracts of a plantdescribed herein and compositions comprising or consisting of suchextracts. In a preferred embodiment, the extract consists of orcomprises tissue of a plant described herein or is obtained from suchtissue.

In still yet another aspect, the invention provides a method ofdetermining the genotype of a plant of the invention comprisingdetecting in the genome (e.g., a sample of nucleic acids) of the plantat least a first polymorphism. The method may, in certain embodiments,comprise detecting a plurality of polymorphisms in the genome of theplant, for example by obtaining a sample of nucleic acid from a plantand detecting in said nucleic acids a plurality of polymorphisms. Themethod may further comprise storing the results of the step of detectingthe plurality of polymorphisms on a computer readable medium.

The invention also provides for a food or feed product comprising orconsisting of a plant part described herein wherein the plant part canbe identified as a part of the plant of the invention. Preferably, theplant part is a onion bulb or part thereof and/or an extract from a bulbor another plant part described herein. The food or feed product may befresh or processed, e.g., dried, grinded, powdered, pickled, chopped,cooked, juiced, preserved, pickled, fried, canned, steamed, boiled,blanched and/or frozen, etc.

For example, containers such as cans, boxes, crates, bags, cartons,Modified Atmosphere Packagings, films (e.g. biodegradable films), etc.comprising plant parts of plants (fresh and/or processed) describedherein are also provided herein.

Marketable onion bulbs are generally sorted by size and quality afterharvest. Alternatively the onion bulbs can be sorted by pungency orsugar content.

Using methods known in the art like “reverse breeding”, it is possibleto produce parental lines for a hybrid plant such as NUN 2002 ON; wherenormally the hybrid is produced from the parental lines. Such methodsare based on the segregation of individual alleles in the sporesproduced by a desired plant and/or in the progeny derived from theself-pollination of that desired plant, and on the subsequentidentification of suitable progeny plants in one generation, or in alimited number of inbred cycles. Such a method is known fromWO2014076249 or from Nature Protocols Volume: 9, Pages: 761-772 (2014)DOI: doi:10.1038/nprot.2014.049, which are enclosed by reference. Suchmethod for producing parental lines for a hybrid organism, comprises thesteps of:

-   a) defining a set of genetic markers that are present in a    heterozygous form (H) in a partially heterozygous starting organism;-   b) producing doubled haploid lines from spores of the starting    organism:-   c) genetically characterizing the doubled haploid lines thus    obtained for the said set of genetic markers to determine whether    they are present in a first homozygous form (A) or in a second    homozygous form (B);-   d) selecting at least one pair of doubled haploid lines that have    complementary alleles for at least a subset of the genetic markers,    wherein each member of the pair is suitable as a parental line for a    hybrid organism.

Thus in one aspect, the invention relates to a method of producing acombination of parental lines of a plant of the invention (NUN 2002 ON)comprising the step of making double haploid cells from haploid cellsfrom the plant of the invention (NUN 2002 ON) or a seed of that plant;and optionally crossing these parental lines to produce and collectseeds. In another aspect, the invention relates to a combination ofparental lines produced by this method. In still another aspect saidcombination of parental lines can be used to produce a seed or plant ofNUN 2002 ON when these parental lines are crossed. In still anotheraspect, the invention relates to a combination of parental lines fromwhich a seed or plant having all but one, two or three physiologicaland/or morphological characteristics of NUN 2002 ON can be produced; orin another aspect, wherein a seed or plant having the distinguishingcharacteristics 1)-5) or 1)-9) of NUN 2002 ON, as herein defined, can beproduced when grown under the same environmental conditions. In stillanother aspect, the invention relates to a combination of parental linesfrom which a seed or plant having all the characteristics of NUN 2002 ONas defined in Table 1 can be produced when grown under the sameconditions.

Deposit Information

A total of 2500 seeds of the hybrid variety NUN 2002 ON were depositedaccording to the Budapest Treaty by Nunhems B.V. on Nov. 21, 2016, atthe NCIMB Ltd., Ferguson Building, Craibstone Estate, Bucksburn,Aberdeen AB21 9YA, United Kingdom (NCIMB). The deposit has been assignedAccession Number NCIMB 42694.

A deposit of NUN 2002 ON and of the male and female parent line is alsomaintained at Nunhems B.V. Access to the deposit will be availableduring the pendency of this application to persons determined by theDirector of the U.S. Patent Office to be entitled thereto upon request.Subject to 37 C.F.R. §1.808(b), all restrictions imposed by thedepositor on the availability to the public of the deposited materialwill be irrevocably removed upon the granting of the patent. The depositwill be maintained for a period of 30 years, or 5 years after the mostrecent request, or for the enforceable life of the patent whichever islonger, and will be replaced if it ever becomes nonviable during thatperiod. Applicant does not waive any rights granted under this patent onthis application or under the Plant Variety Protection Act (7 USC 2321et seq.).

Characteristics of NUN 2002 ON

CARTA BLANCA is considered to be the most similar variety to NUN 2002ON. CARTA BLANCA is a commercial variety bred by Nunhems B.V. In Table 1a comparison between NUN 2002 ON and CARTA BLANCA s shown based on atrial in the USA. Trial location: Bakersfield, Calif., USA (coordinates:35°07452 N, 118°86516 W). Planting date: 12 Nov. 2014.

Two replications of 15 plants each, from which 20 plants or plant partswere randomly selected, were used to measure characteristics. In Table 1the USDA descriptors of NUN 2002 ON (this application) and referenceCARTA BLANCA (commercial variety) are listed.

TABLE 1 Comparison between values* of NUN 2002 ON and CARTA BLANCAApplication Comparison Variety Variety Descriptor NUN 2002 ON CARTABLANCA 1. TYPE: 1 = Bulb 2 = Bunching 1 1 1 = short day; 2 = long day 11 Adaptation range: 10 to 30 10 to 35 Degrees mean latitude Maturity(days) 3 3 1 = early (75-90); 2 = medium (100-120); 3 = late (>130) 2.PLANT: Height above soil line to highest 89 cm 90 cm point of anyfoliage Shorter than comparison variety 1.6 cm — Type: 2 2 1 = erected(Spartan Gem); 2 = intermediate; 3 = floppy (Epoch) 3. LEAF: Length(before maturity 66 cm 67 cm yellowing begins) Width 26 mm 26 mmThickness (at mid-length 2.8 mm 2.6 mm of longest leaf) Color: 2 2 1 =light green (Early Grano); 2 = medium green (Yellow Bermuda); 3 = bluegreen (Australian Brown U.C. No. 1) Color Chart Code (RHS) N137A N137ABloom: 2 2 1 = none-glossy; 2 = light (Early Grano); 3 = medium (CrystalWax); 4 = heavy (California Early Red) 4. SHEATH: Column length (heightfrom 28 mm 40 mm soil line to base of lowest succulent leaf) Diameter(at mid-length) 20.2 mm 17 mm 5. INFLORESCENCE (umbel for seedproduction): Maximum number per plant: 2 3 Minimum number per plant: 1 1Average no. per plant 1 2 Type: 1 2 1 = compact; 2 = loose/open; 3 =shaggy Spathe: 2 2 1 = long beak; 2 = short beak Flower color: 1 1 1 =white; 2 = green; 3 = bright green Anther color: 3 3 1 = light green; 2= dark green; 3 = yellow; 4 = pale yellow; 5 = chocolate; 6 = red Pollenviability 2 2 1 = sterile; 2 = fertile 6. BULB: Average number of bulbsper meter 12  12  Size (harvested) 3 3 1 = small (Red Creol); 2 = medium(Australian Brown U.C. No. 1); 3 = large (Early Grano) Shape 4 4 1 =Globe (White Sweet Spanish); 2 = Deep Globe (Abundance); 3 = Flt. Globe(Australian Brn. U.C. No. 1); 4 = Top Shape (Texas Grano 502); 5 = DeepFlat (Granex); 6 = Thick Flat (Ebenezer); 7 = Flat (Crystal Wax); 8 =Torpedo-Long Oval (Italian Red) Height 10.84 cm 10.07 cm Diameter 10.21cm 9.63 cm Shape Index   1.06   1.05 1 = invaginate; 2 = evaginate n.r.n.r. Color (skin): 09 (RHS 09 (RHS 01 = Brown Greyed Greyed (AustralianBrn. U.C. No. 1); White White 02 = Purplish Red (Italian Red); 156D)154C 03 = Buff Red (Red Creole); 04 = Pinkish Yellow (Ebenezer); 05 =Brownish Yellow (Mt. Danvers); 06 = Deep Yellow (Brigham Yellow Globe);07 = Medium Yellow (Early Yellow Globe); 08 = Pale Yellow (YellowBermuda); 09 = White (White Sweet Spanish); 10 = Other (Specify)         Color (interior) 4 (RHS 4 (RHS 1 = Pink; White White 2 = Red;NN155C) NN155C) 3 = Purplish Red; 4 = White; 5 = Cream; 6 = LightGreen-Yellow; 7 = Dark Green-Yellow Average bulb weight ** 475.1 grams417.5 grams Scales: 1 1 1 = Few (Crystal Wax); 2 = Medium (AustralianBrown U.C. No. 1); 3 = Many (Sweet Spanish) Scales: 3 3 1 = Thick(Australian Brown U.C. No. 1); 2 = Medium (Red Creole); 3 = Thin(Crystal Wax) Scale retention: 3 3 1 = Very Good (Australian Brn. U.S.No. 1); 2 = Good (Ebenezer); 3 = Fair (Red Wethersfield); 4 = Poor(Crystal Wax) Pungency: 2 2 1 = Mild (Early Grano); 2 = Medium (CrystalWax); 3 = Strong (White Creole) Storage: 3 3 1 = Good (Ebenezer); 2 =Fair (Yellow Globe Danvers); 3 = Poor (Crystal Wax) 7. DISEASERESISTANCE 0 = not tested; 1 = susceptible 2 = resistant Black Mold 0 0Neck Mold 0 0 Purple Blotch 0 0 Smut 0 0 Mildew 0 0 Pink root 0 0 Smudge0 0 Yellow dwarf 0 0 8. INSECT RESISTANT 0 = not tested; 1 = susceptible2 = resistant Thrips 0 0 Other (specify) n.r. n.r. *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.N.A. = not applicable; n.r. = not recorded. ** Characteristic is not aUSDA descriptor

REFERENCES

The following references, to the extent that they provide exemplaryprocedural or other details supplementary to those set forth herein, arespecifically incorporated herein by reference:

Choi et al., Plant Cell Rep., 13: 344-348, 1994.

Dunstan and Short (1977) Physiol, Plant, 41: 70-72

SANTOS, C A F et al. Genetic similarity among onion cultivars ofdifferent types and origins, based on AFLP markers. Hortic. Bras.[online]. 2011, vol. 29, n.1, pp. 32-37. ISSN 0102-0536.http://dx.doi.org/10.1590/S0102-05362011000100006.

Pike and Yoo, Scientia Horticulturae, 45 (1990) 31-36

Ellul et al., Theor. Appl. Genet., 107:462-469, 2003.

The invention claimed is:
 1. A seed of onion variety NUN 2002 ON,wherein a representative sample of said seed has been deposited underAccession Number NCIMB
 42694. 2. A plant grown from the seed of claim 1.3. A plant part of the plant of claim 2, further defined as a leaf, abulb, a scale, an ovule, a fruit, a scion, a rootstock, a cutting, aflower or a part of any of these or a cell.
 4. An onion plant, or a partthereof having all the characteristics of the plant of claim 2 as listedin Table
 1. 5. A tissue or cell culture of regenerable cells of theplant of claim
 2. 6. The tissue or cell culture according to claim 5,comprising cells or protoplasts from a plant part selected from thegroup consisting of embryos, meristems, cotyledons, hypocotyl, pollen,leaves, bulbs, scales anthers, roots, root tips, pistil, petiole,flower, fruit, seed, stem and stalks.
 7. An onion plant regenerated fromthe tissue or cell culture of claim 5, wherein the plant has all of thephysiological and morphological characteristics of the plant of claim 2as listed in Table
 1. 8. A method of producing of the plant of claim 2,or a part thereof, comprising vegetative propagation of the plant ofclaim
 2. 9. The method of claim 8, wherein said vegetative propagationcomprises regenerating a whole plant from a part of the plant of claim2.
 10. The method of claim 8, wherein said part is a cutting, a cellculture or a tissue culture.
 11. A vegetative propagated plant of claim2, or a part thereof, wherein the plant has all of the physiological andmorphological characteristics of the plant of claim
 2. 12. A method ofproducing an onion plant, said method comprising crossing the plant ofclaim 2 with a second onion plant one or more times, and selectingprogeny from said crossing and optionally allowing the progeny to formseed.
 13. An onion plant having one physiological and/or morphologicalcharacteristic which is different from those of the plant of claim 2 andwhich otherwise has all the physiological and morphologicalcharacteristics of the plant of claim 2 as listed in Table
 1. 14. A foodor feed product comprising the plant part of claim 3 wherein the plantpart can be identified as a part of the plant of claim
 1. 15. The plantof claim 2 further comprising a single locus conversion, wherein saidplant has otherwise all of the morphological and physiologicalcharacteristics of the plant of claim 2, optionally wherein the singlelocus conversion confers a trait selected from the group consisting ofmale sterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, environmental stress tolerance, modifiedcarbohydrate metabolism and modified protein metabolism.
 16. A plantcomprising the scion or rootstock of claim
 3. 17. A method of producinga combination of parental lines of the plant of claim 2 comprising thestep of making double haploid cells from haploid cells from the plant,plant part or seed of the plant of claim 1; and optionally crossingthese parental lines to produce and collect seeds.